Journal: Frontiers in Endocrinology

Article Title: Transcriptome Analysis Reveal Candidate Genes and Pathways Responses to Lactate Dehydrogenase Inhibition (Oxamate) in Hyperglycemic Human Renal Proximal Epithelial Tubular Cells

doi: 10.3389/fendo.2022.785605

Figure Lengend Snippet: The protein expression levels of peroxisome proliferator-activated receptor-g coactivator 1α (PGC1α), caspase 3 (CASP3), B-cell lymphoma 2 apoptosis regulator (BCL2), BCL2 associated X apoptosis regulator (BAX), beclin1 (BECN1) and microtubule-associated proteins 1A/1B light chain 3 (MAP1LC3) in HK-2 cells treated with low D-glucose (LG) with different concentrations of oxamate (LGOXA-0mM, LGOXA-20mM, LGOXA-40mM and LGOXA-80mM) and high D-glucose (HG) with different concentrations of oxamate (HGOXA-0mM, HGOXA-20mM, HGOXA-40mM and HGOXA-80mM) for 24 hours. (A) Western blot gels. (B) Relative grey values. * indicates P -value < 0.05 after student’s t -test.

Article Snippet: The primary antibodies [Anti-PGC1α (ab54481), Anti-CASP3 (9665), Anti-BCL2 (ab59348), Anti-BAX (GB11690), Anti-BECN1 (bs-1353R), Anti-MAP1LC3 (14600-1-ap) and Anti-β-actin (GB12001)] and all secondary antibodies were purchased from abcom (Cambridge, MA, USA), Proteintech (Wuhan, China), BIOSS (Boston, Massachusetts, USA) and Servicebio (Wuhan, China), respectively.

Techniques: Expressing, Western Blot

Journal: Mediators of Inflammation

Article Title: Guttiferone K Exerts the Anti-inflammatory Effect on Mycobacterium Tuberculosis- (H37Ra-) Infected Macrophages by Targeting the TLR/IRAK-1 Mediated Akt and NF- κ B Pathway

doi: 10.1155/2020/8528901

Figure Lengend Snippet: Guttiferone K restrains NLRP3 inflammasome activity depending on the autophagy process. (a) Western blot analysis of NLRP3, LC3 I/II, and p62 expression in Mtb-infected RAW 264.7 cells after treatment with GK (10 μ M) or CQ (10 or 20 μ M) for 12 hr. (b) Evaluation of si-BECN1 transfection efficiency by Western blot in RAW264.7 cells. (c) BECN1 knockdown downregulates LC3 and restricts the inhibitory effect of GK on NLRP3 in RAW264.7 cells. (d) Western blot analysis of IL-1 β , NLRP3, LC3 I/II, and p62 expression in Mtb-infected primary peritoneal macrophage cells after treatment with different concentrations of GK (5, 10, 20 μ M) or rapamycin (1 μ g/ml). The results are representative of at least three independent experiments.

Article Snippet: The following antibodies were used: anti-NLRP3, anti-IL-1 β , anti-ASC, anti-LC3, anti-p62, anti-Akt, anti-phosphorylated Akt (Ser473), anti-mTOR, anti-phosphorylated mTOR (Ser2448), anti-phosphorylated p38, anti-phosphorylated JNK, and anti-phosphorylated ERK1/2 were purchased from Cell Signaling Technology, Inc. (CST, Danvers, MA, USA); anti-IL-1 β was purchased from R&D Systems (Minnesota, USA); BECN1 siRNA, Transfection Reagents, rabbit anti-caspase-1, goat anti-rabbit, donkey anti-goat, and goat anti-mouse LC3 were purchased from Santa Cruz Biotechnology, Inc.; anti- β -actin monoclonal antibody was from ProteinTech Group (Chicago, IL); goat anti-NLRP3 was purchased from Abcam (Cambridge, UK).

Techniques: Activity Assay, Western Blot, Expressing, Infection, Transfection, Knockdown

Journal: Molecular and cellular biochemistry

Article Title: Early-life bisphenol A exposure causes neuronal pyroptosis in juvenile and adult male rats through the NF-κB/IL-1β/NLRP3/caspase-1 signaling pathway: exploration of age and dose as effective covariates using an in vivo and in silico modeling approach.

doi: 10.1007/s11010-024-05039-4

Figure Lengend Snippet: Fig. 8 The molecular docking analysis of BPA and neuro- inflammatory, autophagic as well as, pyroptotic molecules displaying 2D and 3D binding interactions of BPA [PubChem CID: 6623] against A NF-kB [PDB ID: 1NFK], B IL-1β [PDB ID: 2MIB], C IL-2 [PDB ID: 4YQX], D IL-12 [PDB ID: 3HMX], E COX-2 [PDB ID: 1PXX], F NLRP3 [PDB ID: 7vtq], G Beclin-1 [PDB ID: 2PON], H LC3A [PDB ID: 6TBE], I LC3B [PDB ID: 5XAC], J Caspase-1 [PDB ID: 6VIE]. The green dotted lines denote hydrogen bonds between ligand and aminoacids, whereas bink/purple dotted lines repre- sent hydrophobic interactions. Electrostatic interactions are shown as orange dotted lines. The red dotted line indicate an unfavorable donor-donor

Article Snippet: Fine Test (cat no. ER1965) provided the NLRP3 kit, LSBio (cat no. LS-F9917; LS-F19802) provided the LC3A and LC3B kits, respectively, and Cusabio (cat no. CSB-EL002658RA provided the beclin-1 kit.

Techniques: Binding Assay

Journal: Journal of nanobiotechnology

Article Title: Natural exosome-like nanoparticles derived from ancient medicinal insect Periplaneta americana L. as a novel diabetic wound healing accelerator.

doi: 10.1186/s12951-023-01923-1

Figure Lengend Snippet: Fig. 6 (A) The expression of α-SMA, CD31, VEGF, CD68 and CD90 in the regenerated mouse skin tissue in each group on the 16th day was determined by immunohistochemical staining. (B) Western blot results of MMP-9, BECLIN-1 and p62 protein expression in wound tissue of mice at day 16. *P<0.05 versus DM(+) group

Article Snippet: According to the antibody instructions, the membranes were incubated with P62 (66184-1-Ig, 1:1000, Proteintech Group, Inc.), Beclin-1 (66665-1-Ig, 1:1000, Proteintech Group, Inc.), MMP-9 (GB12132-1, 1:1000, Servicebio) or β-Actin (GB12001, 1:2000, Servicebio).

Techniques: Expressing, Immunohistochemical staining, Staining, Western Blot